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KMID : 0811720080120060337
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Korean Journal of Physiology & Pharmacology
2008 Volume.12 No. 6 p.337 ~ p.342
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Diversity of Ion Channels in Human Bone Marrow Mesenchymal Stem Cells from Amyotrophic Lateral Sclerosis Patients
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Park Kyoung-Sun
Kim Hyun-Young
Kim Seung-Hyun
Kim Yang-Mi
Cha Eun-Jong
Jung Kyoung-Hwa
CHoi Mi-Ran
Kim Kyung-Suk
Chai Young-Gyu
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Abstract
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Human bone marrow mesenchymal stem cells (hBM-MSCs) represent a potentially valuable cell type for clinical therapeutic applications. The present study was designed to evaluate the effect of long-term culturing (up to 10th passages) of hBM-MSCs from eight individual amyotrophic lateral sclerosis (ALS) patients, focusing on functional ion channels. All hBM-MSCs contain several MSCs markers with no significant differences, whereas the distribution of functional ion channels was shown to be different between cells. Four types of K＋ currents, including noise-like Ca＋2-activated K＋ current (IKCa), a transient outward K＋ current (Ito), a delayed rectifier K＋ current (IKDR), and an inward-rectifier K＋ current (Kir) were heterogeneously present in these cells, and a TTX-sensitive Na＋ current (INa,TTX) was also recorded. In the RT-PCR analysis, Kv1.1, heag1, Kv4.2, Kir2.1, MaxiK, and hNE-Na were detected. In particular, INa,TTX showed a significant passage-dependent increase. This is the first report showing that functional ion channel profiling depend on the cellular passage of hBM-MSCs.
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KEYWORD
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Bone marrow, Stem cells, Functional ion channels, Tetrodotoxin-sensitive Na＋ current, Passage-dependency
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